0.2 mL 96-Well Semi-Skirted PCR Plate, Straight-Side Frame

0.2 mL 96-Well Semi-Skirted PCR Plate, Straight-Side Frame for Consistent PCR and qPCR Workflows

The Puretest 0.2 mL 96-well semi-skirted PCR plate uses a straight-sided frame for stable bench handling, sealing and selected automation carriers. Standard-height thin-wall wells suit routine endpoint PCR and compatible real-time PCR workflows.

The plate combines a reinforced semi-skirt frame with straight outer sidewalls and a standard 8 × 12 well array. The frame improves stability while leaving portions of the wells accessible for block contact and handling.

Product Highlights

• Straight-sided semi skirt: Provides a predictable outer profile for carriers and manual handling.
• 0.2 mL standard-height wells: Accommodate common PCR reaction volumes.
• High-purity polypropylene wells: Support rapid thermal transfer and low nonspecific binding.
• Uniform thin-wall molding: Promotes consistent heating and cooling from well to well.
• Raised sealing rims: Help films or heat seals close each reaction independently.
• Alphanumeric indexing: Supports plate maps, sample identification and orientation.
• Clear and white options: Select optical configuration according to the PCR or qPCR detection method.
• Automation-oriented supply: Technical drawings, samples, barcode and private-label options are available.

Plate Structure and Functional Design

The plate combines a reinforced semi-skirt frame with straight outer sidewalls and a standard 8 × 12 well array. The frame improves stability while leaving portions of the wells accessible for block contact and handling.

Use a flat support when sealing or transporting the plate. Check that the straight sidewalls do not interfere with the instrument carrier or robotic gripper.

Technical Specifications

Dimensions, working volumes and compatibility are nominal unless stated in the current Puretest drawing. Validate the exact instrument, seal and thermal protocol before routine use.

Instrument Compatibility and Selection Guide

The straight-side frame is suitable for selected universal-style carriers and thermal cyclers, but exact support differs by instrument. Confirm the block recess, skirt clearance, corner cut and heated-lid geometry.

• Confirm the number of wells, block height, skirt style, corner cut and notch position.
• For qPCR, confirm whether clear or white wells and an optical seal are required.
• For automation, validate gripper access, deck carrier, barcode area and plate rigidity.
• Request a physical sample before releasing a new plate into a validated assay.

Application Guide

Sealing, Thermal Cycling and Handling

• Use an optical adhesive film, heat seal or cap solution compatible with the plate and detection method.
• Apply uniform sealing pressure and confirm that every well rim is fully closed before cycling.
• Centrifuge briefly when the protocol requires liquid collection at the well bottom, using a supported carrier.
• Keep wells and sealing rims free from powder, oil and fingerprints.
• Nominal well capacity is not the recommended reaction volume; preserve headspace and follow the assay protocol.

Material, Cleanliness and Quality Documentation

Puretest laboratory consumables are molded from high-purity polypropylene selected for chemical resistance, low biomolecule binding and dependable dimensional consistency. Available cleanliness, sterile and packaging configurations must be matched to the quoted catalog number.

If your workflow requires a Certificate of Analysis, Certificate of Conformance, irradiation record, DNase/RNase status, pyrogen limit, human-DNA limit, dimensional report or batch traceability, state the requirement before ordering. Puretest will confirm which documents apply to the selected configuration rather than treating every supply option as identical.

Ordering Information, Packaging and OEM Support

Puretest supports evaluation samples, current technical drawings, bulk and individually protected packaging, sterile and non-sterile options, customer catalog numbers, private labels, barcodes and distributor carton programs. MOQ, inner-pack quantity, case quantity, lead time, carton dimensions and shipping method are confirmed with the final specification.

Choose the Right PCR Plate Format

Instrument names describe compatibility style only. Confirm the block format, skirt, corner cut, notch position, optical requirement and current drawing before ordering.

Trademark and Compatibility Notice

ABI®, Bio-Rad®, FAST®, LightCycler® and Roche® are trademarks of their respective owners. Puretest is not affiliated with or endorsed by those companies. Compatibility-style descriptions help buyers identify frame geometry and do not replace instrument validation.

Frequently Asked Questions

What is the advantage of straight sides?

They provide a consistent external surface for manual handling and selected carriers.

Is it the same as the ABI®-type semi-skirted plate?

No. The external frame and deck geometry can differ even though both use 0.2 mL wells.

Can it be used in a qPCR instrument?

Only when the block, optics, plate color and seal are compatible.

What reaction volume should I use?

Follow the assay and instrument guidance; nominal capacity is not the recommended reaction volume.

Are samples available for fitting tests?

Yes. Send the carrier or instrument model and desired plate color.

Request a Sample, Drawing or Quotation

Tell us the required 0.2 mL semi-skirted straight side, thermal cycler model, reaction volume, optical color, skirt and corner/notch requirement, annual quantity and destination country. Contact Puretest for a sample, current technical drawing, cleanliness-document confirmation or commercial quotation.