0.2 mL 96-Well Semi-Skirted PCR Plate, ABI®-Type

0.2 mL 96-Well Semi-Skirted PCR Plate, ABI®-Type for Consistent PCR and qPCR Workflows

The Puretest 0.2 mL 96-well semi-skirted ABI®-type PCR plate combines standard-height reaction wells with a stable raised deck. It is intended for compatible ABI-type thermal cyclers, real-time PCR systems and sequencer workflows after exact model validation.

The semi skirt reinforces the 96-well array while leaving selected sides accessible. Standard-height thin-wall wells support routine PCR volumes, and the frame includes corner and deck features intended to align with compatible ABI-type blocks.

Product Highlights

• 0.2 mL standard-height wells: Support common PCR reaction volumes.
• ABI®-type semi-skirt frame: Provides stable handling and instrument-oriented geometry.
• High-purity polypropylene wells: Support rapid thermal transfer and low nonspecific binding.
• Uniform thin-wall molding: Promotes consistent heating and cooling from well to well.
• Raised sealing rims: Help films or heat seals close each reaction independently.
• Alphanumeric indexing: Supports plate maps, sample identification and orientation.
• Clear and white options: Select optical configuration according to the PCR or qPCR detection method.
• Automation-oriented supply: Technical drawings, samples, barcode and private-label options are available.

Plate Structure and Functional Design

The semi skirt reinforces the 96-well array while leaving selected sides accessible. Standard-height thin-wall wells support routine PCR volumes, and the frame includes corner and deck features intended to align with compatible ABI-type blocks.

Seat the semi skirt evenly in the carrier and confirm that the heated lid applies uniform pressure to the seal without deforming the wells.

Technical Specifications

Dimensions, working volumes and compatibility are nominal unless stated in the current Puretest drawing. Validate the exact instrument, seal and thermal protocol before routine use.

Instrument Compatibility and Selection Guide

Designed around selected ABI®-type 0.2 mL blocks and sequencer-compatible carriers. Confirm instrument generation, corner cut, raised-deck height and optical requirements before ordering.

• Confirm the number of wells, block height, skirt style, corner cut and notch position.
• For qPCR, confirm whether clear or white wells and an optical seal are required.
• For automation, validate gripper access, deck carrier, barcode area and plate rigidity.
• Request a physical sample before releasing a new plate into a validated assay.

Application Guide

Sealing, Thermal Cycling and Handling

• Use an optical adhesive film, heat seal or cap solution compatible with the plate and detection method.
• Apply uniform sealing pressure and confirm that every well rim is fully closed before cycling.
• Centrifuge briefly when the protocol requires liquid collection at the well bottom, using a supported carrier.
• Keep wells and sealing rims free from powder, oil and fingerprints.
• Nominal well capacity is not the recommended reaction volume; preserve headspace and follow the assay protocol.

Material, Cleanliness and Quality Documentation

Puretest laboratory consumables are molded from high-purity polypropylene selected for chemical resistance, low biomolecule binding and dependable dimensional consistency. Available cleanliness, sterile and packaging configurations must be matched to the quoted catalog number.

If your workflow requires a Certificate of Analysis, Certificate of Conformance, irradiation record, DNase/RNase status, pyrogen limit, human-DNA limit, dimensional report or batch traceability, state the requirement before ordering. Puretest will confirm which documents apply to the selected configuration rather than treating every supply option as identical.

Ordering Information, Packaging and OEM Support

Puretest supports evaluation samples, current technical drawings, bulk and individually protected packaging, sterile and non-sterile options, customer catalog numbers, private labels, barcodes and distributor carton programs. MOQ, inner-pack quantity, case quantity, lead time, carton dimensions and shipping method are confirmed with the final specification.

Choose the Right PCR Plate Format

Instrument names describe compatibility style only. Confirm the block format, skirt, corner cut, notch position, optical requirement and current drawing before ordering.

Trademark and Compatibility Notice

ABI®, Bio-Rad®, FAST®, LightCycler® and Roche® are trademarks of their respective owners. Puretest is not affiliated with or endorsed by those companies. Compatibility-style descriptions help buyers identify frame geometry and do not replace instrument validation.

Frequently Asked Questions

Does ABI®-type mean it fits all Applied Biosystems instruments?

No. It describes a compatibility family. Exact block, deck and corner geometry vary by instrument.

When should I choose 0.2 mL instead of 0.1 mL?

Choose 0.2 mL for standard-height blocks and protocols that need greater reaction or headspace volume.

Can it be used for qPCR?

Yes when the plate color, optical seal and instrument are compatible.

Is the frame suitable for robotics?

The semi skirt improves handling, but gripper and carrier validation are still required.

Can I obtain a drawing?

Yes. Provide the instrument model and intended seal when requesting it.

Request a Sample, Drawing or Quotation

Tell us the required 0.2 mL semi-skirted ABI®-type, thermal cycler model, reaction volume, optical color, skirt and corner/notch requirement, annual quantity and destination country. Contact Puretest for a sample, current technical drawing, cleanliness-document confirmation or commercial quotation.