0.1 mL 96-Well Semi-Skirted PCR Plate, LightCycler®-Type

0.1 mL 96-Well Semi-Skirted PCR Plate, LightCycler®-Type for Consistent PCR and qPCR Workflows

The Puretest 0.1 mL 96-well semi-skirted LightCycler®-type PCR plate combines low-profile thin-wall wells with an instrument-oriented notched frame. It is intended for compatible real-time PCR blocks after exact model and corner validation.

The semi-skirt frame includes orientation features that distinguish it from general 96-well PCR plates. The notch and corner pattern must align with the carrier, while thin-wall low-profile wells provide close block contact for thermal transfer.

Product Highlights

• Instrument-oriented notch pattern: Supports correct placement in selected LightCycler®-type carriers.
• 0.1 mL low-profile wells: Reduce dead space and support efficient optical thermal cycling.
• High-purity polypropylene wells: Support rapid thermal transfer and low nonspecific binding.
• Uniform thin-wall molding: Promotes consistent heating and cooling from well to well.
• Raised sealing rims: Help films or heat seals close each reaction independently.
• Alphanumeric indexing: Supports plate maps, sample identification and orientation.
• Clear and white options: Select optical configuration according to the PCR or qPCR detection method.
• Automation-oriented supply: Technical drawings, samples, barcode and private-label options are available.

Plate Structure and Functional Design

The semi-skirt frame includes orientation features that distinguish it from general 96-well PCR plates. The notch and corner pattern must align with the carrier, while thin-wall low-profile wells provide close block contact for thermal transfer.

Align the notches and corner orientation before closing the instrument lid. Never force a misaligned plate into the carrier, as this may deform the wells or compromise optical contact.

Technical Specifications

Dimensions, working volumes and compatibility are nominal unless stated in the current Puretest drawing. Validate the exact instrument, seal and thermal protocol before routine use.

Instrument Compatibility and Selection Guide

The notch pattern and skirt geometry are designed around selected LightCycler®-type instruments. Confirm whether your system is LightCycler 480, LightCycler 96 or another model; block and carrier requirements are not interchangeable.

• Confirm the number of wells, block height, skirt style, corner cut and notch position.
• For qPCR, confirm whether clear or white wells and an optical seal are required.
• For automation, validate gripper access, deck carrier, barcode area and plate rigidity.
• Request a physical sample before releasing a new plate into a validated assay.

Application Guide

Sealing, Thermal Cycling and Handling

• Use an optical adhesive film, heat seal or cap solution compatible with the plate and detection method.
• Apply uniform sealing pressure and confirm that every well rim is fully closed before cycling.
• Centrifuge briefly when the protocol requires liquid collection at the well bottom, using a supported carrier.
• Keep wells and sealing rims free from powder, oil and fingerprints.
• Nominal well capacity is not the recommended reaction volume; preserve headspace and follow the assay protocol.

Material, Cleanliness and Quality Documentation

Puretest laboratory consumables are molded from high-purity polypropylene selected for chemical resistance, low biomolecule binding and dependable dimensional consistency. Available cleanliness, sterile and packaging configurations must be matched to the quoted catalog number.

If your workflow requires a Certificate of Analysis, Certificate of Conformance, irradiation record, DNase/RNase status, pyrogen limit, human-DNA limit, dimensional report or batch traceability, state the requirement before ordering. Puretest will confirm which documents apply to the selected configuration rather than treating every supply option as identical.

Ordering Information, Packaging and OEM Support

Puretest supports evaluation samples, current technical drawings, bulk and individually protected packaging, sterile and non-sterile options, customer catalog numbers, private labels, barcodes and distributor carton programs. MOQ, inner-pack quantity, case quantity, lead time, carton dimensions and shipping method are confirmed with the final specification.

Choose the Right PCR Plate Format

Instrument names describe compatibility style only. Confirm the block format, skirt, corner cut, notch position, optical requirement and current drawing before ordering.

Trademark and Compatibility Notice

ABI®, Bio-Rad®, FAST®, LightCycler® and Roche® are trademarks of their respective owners. Puretest is not affiliated with or endorsed by those companies. Compatibility-style descriptions help buyers identify frame geometry and do not replace instrument validation.

Frequently Asked Questions

Why does the notch pattern matter?

It prevents incorrect orientation and matches instrument-specific carrier geometry.

Will it fit every Roche® real-time PCR instrument?

No. Verify the exact model and current drawing.

Should I use white wells?

White wells are commonly selected for qPCR fluorescence control, but follow the instrument and assay recommendations.

Can a standard 96-well seal be used?

A compatible 96-well optical film may be suitable, but validate adhesive, optical clarity and notch clearance.

Are barcodes available?

Barcode and private-label options can be discussed for the final packaging configuration.

Request a Sample, Drawing or Quotation

Tell us the required 0.1 mL semi-skirted LightCycler®-type, thermal cycler model, reaction volume, optical color, skirt and corner/notch requirement, annual quantity and destination country. Contact Puretest for a sample, current technical drawing, cleanliness-document confirmation or commercial quotation.