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0.1 mL 96-Well Semi-Skirted PCR Plate, FAST®-Type

The Puretest 0.1 mL 96-well semi-skirted FAST®-type PCR plate is designed for low-profile rapid-cycling blocks. The semi skirt balances frame rigidity with accessible well sides for manual and automated handling.

  • 0.1 mL fast-cycling profile: Low well mass and reduced headspace support rapid temperature changes.
  • Semi-skirted frame: Provides handling rigidity while retaining side access.
  • High-purity polypropylene wells: Support rapid thermal transfer and low nonspecific binding.
  • Uniform thin-wall molding: Promotes consistent heating and cooling from well to well.
  • Raised sealing rims: Help films or heat seals close each reaction independently.

Request a sample, drawing or quotation

PRODUCT DETAILS

0.1 mL 96-Well Semi-Skirted PCR Plate, FAST®-Type for Consistent PCR and qPCR Workflows

The Puretest 0.1 mL 96-well semi-skirted FAST®-type PCR plate is designed for low-profile rapid-cycling blocks. The semi skirt balances frame rigidity with accessible well sides for manual and automated handling.

The 8 × 12 well array uses low-profile thin-wall tubes connected to a semi skirt. This construction is lighter than a full-skirt frame but more rigid than a non-skirted plate, making it useful for routine handling and selected automated workflows.

Puretest 96-well and 384-well PCR plate family
Representative Puretest PCR plate structures, including full-skirted, semi-skirted, non-skirted and 384-well formats.

Product Highlights

  • 0.1 mL fast-cycling profile: Low well mass and reduced headspace support rapid temperature changes.
  • Semi-skirted frame: Provides handling rigidity while retaining side access.
  • High-purity polypropylene wells: Support rapid thermal transfer and low nonspecific binding.
  • Uniform thin-wall molding: Promotes consistent heating and cooling from well to well.
  • Raised sealing rims: Help films or heat seals close each reaction independently.
  • Alphanumeric indexing: Supports plate maps, sample identification and orientation.
  • Clear and white options: Select optical configuration according to the PCR or qPCR detection method.
  • Automation-oriented supply: Technical drawings, samples, barcode and private-label options are available.

Plate Structure and Functional Design

The 8 × 12 well array uses low-profile thin-wall tubes connected to a semi skirt. This construction is lighter than a full-skirt frame but more rigid than a non-skirted plate, making it useful for routine handling and selected automated workflows.

Keep reaction volumes below the nominal well capacity and apply uniform sealing pressure. Confirm that the semi skirt sits flat without rocking in the instrument carrier.

Technical Specifications

Plate format 96 wells; 8 × 12 array
Nominal well format 0.1 mL low profile
Typical reaction-volume reference Approximately 10–100 µL; follow assay and instrument guidance
Skirt style Semi skirt
Compatibility style FAST®-type low-profile block; verify exact instrument
Material High-purity polypropylene (PP)
Well wall Uniform thin wall
Pitch 9 mm nominal
Color options Clear and white subject to quotation
Sealing Optical adhesive film or compatible heat seal
Cleanliness Applicable DNase/RNase, pyrogen and PCR-inhibitor status confirmed for quoted SKU

Dimensions, working volumes and compatibility are nominal unless stated in the current Puretest drawing. Validate the exact instrument, seal and thermal protocol before routine use.

Instrument Compatibility and Selection Guide

Designed around selected 0.1 mL fast-cycling block geometries. FAST® is a compatibility-style reference and does not guarantee fit across every instrument generation. Verify block depth, skirt clearance, corner cut and heated lid.

  • Confirm the number of wells, block height, skirt style, corner cut and notch position.
  • For qPCR, confirm whether clear or white wells and an optical seal are required.
  • For automation, validate gripper access, deck carrier, barcode area and plate rigidity.
  • Request a physical sample before releasing a new plate into a validated assay.

Application Guide

Application Why This Format Is Suitable
Fast PCR Low-profile wells support rapid heating and cooling in compatible blocks.
qPCR / real-time PCR Optical configurations support fluorescence workflows after validation.
High-throughput genotyping Semi-skirted frame supports traceable plate maps and handling.
Automated reaction setup Frame can be integrated after gripper and carrier validation.
Molecular screening 96-well format supports parallel amplification of multiple samples.

Sealing, Thermal Cycling and Handling

  • Use an optical adhesive film, heat seal or cap solution compatible with the plate and detection method.
  • Apply uniform sealing pressure and confirm that every well rim is fully closed before cycling.
  • Centrifuge briefly when the protocol requires liquid collection at the well bottom, using a supported carrier.
  • Keep wells and sealing rims free from powder, oil and fingerprints.
  • Nominal well capacity is not the recommended reaction volume; preserve headspace and follow the assay protocol.

Material, Cleanliness and Quality Documentation

Puretest laboratory consumables are molded from high-purity polypropylene selected for chemical resistance, low biomolecule binding and dependable dimensional consistency. Available cleanliness, sterile and packaging configurations must be matched to the quoted catalog number.

If your workflow requires a Certificate of Analysis, Certificate of Conformance, irradiation record, DNase/RNase status, pyrogen limit, human-DNA limit, dimensional report or batch traceability, state the requirement before ordering. Puretest will confirm which documents apply to the selected configuration rather than treating every supply option as identical.

Ordering Information, Packaging and OEM Support

Puretest supports evaluation samples, current technical drawings, bulk and individually protected packaging, sterile and non-sterile options, customer catalog numbers, private labels, barcodes and distributor carton programs. MOQ, inner-pack quantity, case quantity, lead time, carton dimensions and shipping method are confirmed with the final specification.

Choose the Right PCR Plate Format

Plate Format Well Count Frame / Orientation Best-Fit Workflow
40 µL 384-well one-notch 384 wells ABI®-type one-notch frame High-throughput low-volume PCR/qPCR
40 µL 384-well two-notch 384 wells Roche®-type two-notch frame Selected Roche®-type 384-well systems
0.1 mL full-skirted 96 wells Bio-Rad®-type frame Rigid low-profile PCR/qPCR workflows
0.1 mL semi-skirted FAST®-type 96 wells Low-profile semi skirt Fast-cycling block formats
0.1 mL semi-skirted LightCycler®-type 96 wells Notched semi skirt Selected LightCycler®-type instruments
0.2 mL non-skirted 96 wells Flexible non-skirted frame Broad routine PCR compatibility
0.2 mL semi-skirted ABI®-type 96 wells Raised semi skirt Selected ABI®-type blocks and automation
0.2 mL semi-skirted straight side 96 wells Straight-sided semi skirt Routine PCR and plate handling

Instrument names describe compatibility style only. Confirm the block format, skirt, corner cut, notch position, optical requirement and current drawing before ordering.

Trademark and Compatibility Notice

ABI®, Bio-Rad®, FAST®, LightCycler® and Roche® are trademarks of their respective owners. Puretest is not affiliated with or endorsed by those companies. Compatibility-style descriptions help buyers identify frame geometry and do not replace instrument validation.

Frequently Asked Questions

What makes this a FAST®-type plate?

It uses a low-profile 0.1 mL geometry associated with rapid-cycling blocks. Instrument-specific dimensions must still be checked.

Why choose a semi skirt?

It adds rigidity and handling surfaces without enclosing the entire plate perimeter.

Can I use it for qPCR?

Yes when the well color, seal and instrument optics are compatible.

Does it fit standard 0.2 mL blocks?

Not necessarily. Low-profile 0.1 mL plates and 0.2 mL plates differ in height and block geometry.

Can I request a sample?

Yes. Provide the exact instrument model and reaction volume.

Request a Sample, Drawing or Quotation

Tell us the required 0.1 mL semi-skirted FAST®-type, thermal cycler model, reaction volume, optical color, skirt and corner/notch requirement, annual quantity and destination country. Contact Puretest for a sample, current technical drawing, cleanliness-document confirmation or commercial quotation.

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