40 µL 384-Well PCR Plate, ABI®-Type, One-Notch

40 µL 384-Well PCR Plate, ABI®-Type, One-Notch for Consistent PCR and qPCR Workflows

The Puretest 40 µL 384-well one-notch PCR plate provides high-density low-volume amplification in an ABI®-type full-skirted frame. Its 4.5 mm well pitch and rigid deck support high-throughput PCR, qPCR and automated reaction setup after instrument validation.

Three hundred eighty-four thin-wall wells are arranged at 4.5 mm pitch within a full-skirt frame. The one-notch corner/orientation pattern is designed around selected ABI-type 384-well carriers. High-density geometry demands accurate pipetting and a flat, rigid deck.

Product Highlights

• 384-well high-density array: Quadruples throughput relative to a 96-well plate in the same general footprint.
• 40 µL nominal wells: Support low-volume reactions and reduced reagent consumption.
• ABI®-type one-notch orientation: Helps identify the required instrument and loading direction.
• Rigid full skirt: Supports robotic handling, stacking and barcode placement.
• High-purity polypropylene wells: Support rapid thermal transfer and low nonspecific binding.
• Uniform thin-wall molding: Promotes consistent heating and cooling from well to well.
• Raised sealing rims: Help films or heat seals close each reaction independently.
• Alphanumeric indexing: Supports plate maps, sample identification and orientation.
• Clear and white options: Select optical configuration according to the PCR or qPCR detection method.
• Automation-oriented supply: Technical drawings, samples, barcode and private-label options are available.

Plate Structure and Functional Design

Three hundred eighty-four thin-wall wells are arranged at 4.5 mm pitch within a full-skirt frame. The one-notch corner/orientation pattern is designed around selected ABI-type 384-well carriers. High-density geometry demands accurate pipetting and a flat, rigid deck.

Use calibrated 384-channel or low-volume dispensing equipment where possible. Apply uniform sealing pressure and centrifuge in a fully supported 384-well carrier.

Technical Specifications

Dimensions, working volumes and compatibility are nominal unless stated in the current Puretest drawing. Validate the exact instrument, seal and thermal protocol before routine use.

Instrument Compatibility and Selection Guide

Designed around selected ABI®-type 384-well blocks using a one-notch orientation. Confirm the exact instrument, notch position, corner cut, plate color and optical seal. A one-notch plate should not be substituted for a two-notch Roche®-type frame without validation.

• Confirm the number of wells, block height, skirt style, corner cut and notch position.
• For qPCR, confirm whether clear or white wells and an optical seal are required.
• For automation, validate gripper access, deck carrier, barcode area and plate rigidity.
• Request a physical sample before releasing a new plate into a validated assay.

Application Guide

Sealing, Thermal Cycling and Handling

• Use an optical adhesive film, heat seal or cap solution compatible with the plate and detection method.
• Apply uniform sealing pressure and confirm that every well rim is fully closed before cycling.
• Centrifuge briefly when the protocol requires liquid collection at the well bottom, using a supported carrier.
• Keep wells and sealing rims free from powder, oil and fingerprints.
• Nominal well capacity is not the recommended reaction volume; preserve headspace and follow the assay protocol.

Material, Cleanliness and Quality Documentation

Puretest laboratory consumables are molded from high-purity polypropylene selected for chemical resistance, low biomolecule binding and dependable dimensional consistency. Available cleanliness, sterile and packaging configurations must be matched to the quoted catalog number.

If your workflow requires a Certificate of Analysis, Certificate of Conformance, irradiation record, DNase/RNase status, pyrogen limit, human-DNA limit, dimensional report or batch traceability, state the requirement before ordering. Puretest will confirm which documents apply to the selected configuration rather than treating every supply option as identical.

Ordering Information, Packaging and OEM Support

Puretest supports evaluation samples, current technical drawings, bulk and individually protected packaging, sterile and non-sterile options, customer catalog numbers, private labels, barcodes and distributor carton programs. MOQ, inner-pack quantity, case quantity, lead time, carton dimensions and shipping method are confirmed with the final specification.

Choose the Right PCR Plate Format

Instrument names describe compatibility style only. Confirm the block format, skirt, corner cut, notch position, optical requirement and current drawing before ordering.

Trademark and Compatibility Notice

ABI®, Bio-Rad®, FAST®, LightCycler® and Roche® are trademarks of their respective owners. Puretest is not affiliated with or endorsed by those companies. Compatibility-style descriptions help buyers identify frame geometry and do not replace instrument validation.

Frequently Asked Questions

Why choose a 384-well plate?

It increases throughput and reduces reaction volume, but requires more precise dispensing and instrument matching.

What does one-notch mean?

It describes an orientation feature in the skirt. The notch must match the carrier and instrument geometry.

Can it replace a Roche®-type two-notch plate?

Not automatically. The skirt and orientation features differ.

What reaction volume is typical?

Many 384-well assays use approximately 5–20 µL, but follow the validated assay and instrument limits.

Which seal is required?

Use a 384-well optical film or compatible heat seal that maintains closure across the full high-density array.

Request a Sample, Drawing or Quotation

Tell us the required 40 µL 384-well one-notch, thermal cycler model, reaction volume, optical color, skirt and corner/notch requirement, annual quantity and destination country. Contact Puretest for a sample, current technical drawing, cleanliness-document confirmation or commercial quotation.