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200 µL 96-Well Square V-Bottom Elution Plate

The Puretest 96-well square V-bottom elution plate is designed for low-volume sample recovery, especially approximately 200 µL nucleic-acid elution and magnetic-bead workflows. Its conical wells concentrate liquid at the lowest point for efficient aspiration while retaining a standard automation-friendly plate footprint.

  • 96 square wells with conical V bottoms: Direct liquid toward a defined aspiration point to help reduce residual volume.
  • Approximately 200 µL elution workflow: Suited to final eluate collection after magnetic-bead washing and separation steps.
  • Approximately 0.5 mL nominal well capacity: Provides headspace around a 200 µL process volume for robotic dispensing and handling.
  • Natural polypropylene construction: Supports chemical resistance and low nonspecific biomolecule binding.
  • ANSI/SLAS-style footprint: Supports multichannel pipettes and selected automated extraction or liquid-handling systems.

Request a sample, technical drawing or quotation

PRODUCT DETAILS

200 µL 96-Well Square V-Bottom Elution Plate for Reliable Sample Processing and Storage

The Puretest 96-well square V-bottom elution plate is designed for low-volume sample recovery, especially approximately 200 µL nucleic-acid elution and magnetic-bead workflows. Its conical wells concentrate liquid at the lowest point for efficient aspiration while retaining a standard automation-friendly plate footprint.

Each of the 96 square wells terminates in a steep V-shaped cone. Unlike a general-purpose 2 mL deep-well plate, this format is optimized around small eluate volumes: the cone gathers liquid for bottom-position aspiration, while the upper well volume provides process headspace. It is an elution and collection plate, not a PCR cycling plate unless separately validated for that use.

Product Highlights

  • 96 square wells with conical V bottoms: Direct liquid toward a defined aspiration point to help reduce residual volume.
  • Approximately 200 µL elution workflow: Suited to final eluate collection after magnetic-bead washing and separation steps.
  • Approximately 0.5 mL nominal well capacity: Provides headspace around a 200 µL process volume for robotic dispensing and handling.
  • Natural polypropylene construction: Supports chemical resistance and low nonspecific biomolecule binding.
  • ANSI/SLAS-style footprint: Supports multichannel pipettes and selected automated extraction or liquid-handling systems.
  • Alphanumeric indexing and corner orientation: Improve sample tracking and deck placement.
  • Sealing options: Compatible sealing film or a geometry-matched mat can be selected for storage and transport.
  • OEM supply support: Samples, sterile options, private labels and customized packaging can be discussed.

Plate Structure and Functional Design

Each of the 96 square wells terminates in a steep V-shaped cone. Unlike a general-purpose 2 mL deep-well plate, this format is optimized around small eluate volumes: the cone gathers liquid for bottom-position aspiration, while the upper well volume provides process headspace. It is an elution and collection plate, not a PCR cycling plate unless separately validated for that use.

For magnetic-particle workflows, confirm the tip comb, magnet position, aspirate height and deck carrier before validation. Set the liquid-level and bottom-clearance parameters conservatively during initial automation trials to avoid contacting the V-bottom apex.

Technical Specifications

Plate format 96 wells; 8 × 12 array
Intended process volume Approximately 200 µL elution workflows
Nominal well capacity Approximately 0.5 mL per well
Well geometry Square well; conical V bottom
Material Natural high-purity polypropylene (PP)
Surface Non-treated
Footprint ANSI/SLAS-style microplate footprint
Orientation Alphanumeric well identification and corner index
Sealing Compatible adhesive film, heat seal or square-well sealing mat; confirm selection
Sterility Sterile and non-sterile supply options subject to quotation
Cleanliness Applicable DNase/RNase and pyrogen status confirmed for the quoted SKU
Use Single use; laboratory research and validated sample-processing workflows

Values are nominal unless a tolerance is stated in the current Puretest drawing. Confirm dimensions, working-volume limits, packaging and applicable quality documentation before equipment validation or a production order.

Application Guide

Application Why This Format Is Suitable
Magnetic-bead nucleic-acid extraction Collects low-volume eluate after wash and separation steps.
DNA/RNA elution V-bottom concentrates the final liquid for controlled aspiration.
Automated sample preparation Standard footprint and 96-position array support parallel processing.
Low-volume reagent transfer Square wells provide access while the cone improves bottom recovery.
Short-term sample holding Compatible seals can protect samples between validated workflow steps.

Workflow and Equipment Compatibility

  • Manual single-channel and multichannel pipetting
  • Automated liquid handlers, plate movers and stackers using a compatible carrier
  • Selected centrifuge carriers and microplate shakers after mechanical validation
  • Adhesive film, heat-sealing film and geometry-matched silicone sealing mats
  • Cold storage, sample preparation and extraction workflows within the validated SKU limits

Material, Cleanliness and Quality Documentation

The plate is manufactured from natural high-purity polypropylene selected for chemical resistance, low biomolecule binding and reliable molding consistency. Available cleanliness and supply configurations should be defined in the quotation. Puretest can confirm the applicable DNase/RNase, pyrogen, sterility and lot-document status for the selected catalog configuration.

If your workflow requires a Certificate of Analysis, Certificate of Conformance, irradiation record, dimensional report, extractables information or a specific contaminant limit, state the requirement before ordering. This ensures that the quoted SKU and batch documentation match your laboratory or regulated purchasing specification.

Sealing, Storage and Handling

  • Use a compatible adhesive film, heat-sealing film or silicone sealing mat selected for the well geometry.
  • Keep the sealing surface clean and dry; confirm plate and sealer settings before transport or long-term storage.
  • For validated autoclavable configurations, use 121°C for 20 minutes and allow the plate to cool without mechanical load. Confirm the current SKU before autoclaving.
  • Leave adequate headspace for mixing, shaking, thermal expansion and sealing; nominal well capacity is not the recommended working volume.
  • Confirm the supported temperature and centrifugation limits for the selected SKU, rotor, carrier and fill volume.
  • Inspect the plate and package before use. Do not use a sterile product if its package is open, wet, punctured or otherwise damaged.

Ordering Information, Packaging and OEM Support

The plate footprint is designed around common ANSI/SLAS microplate conventions for use with multichannel pipettes, plate handlers and automated liquid-handling platforms. Compatibility still depends on the instrument carrier, gripper, deck position, plate height, tip penetration and software settings; validate the current drawing and a physical sample before routine automation.

Puretest supports samples, technical drawings, sterile and non-sterile supply options, private labels, customer catalog numbers, barcodes, customized pouch and carton artwork, and distributor packaging programs. MOQ, case quantity, carton dimensions, lead time and international shipping method are confirmed with the selected configuration.

Choose the Right Deep Well Plate Format

Nominal Format Well Geometry Best-Fit Workflow
200 µL elution 96-well square V-bottom Low-volume nucleic-acid elution and magnetic-bead workflows
1.2 mL 96-well round U-bottom Resuspension, sample processing and compact storage
1.6 mL 96-well square U-bottom Higher capacity in an automation-friendly footprint
2.0 mL 96-well round U-bottom Mixing, recovery and general sample processing
2.2 mL 96-well U- and V-bottom options Magnetic-bead processing, high-volume storage and extraction
2.3 mL 96-well square diamond bottom Low-dead-volume mixing, automated extraction and high-volume sample processing
4.6 mL 48-well square U-bottom Larger-volume culture, mixing and sample preparation

Choose by required working volume, residual-volume target, mixing method, sealing method and instrument carrier. Request the current drawing before validating an automated protocol.

Frequently Asked Questions

Why is it called a 200 µL plate if the nominal well capacity is larger?

The larger nominal cavity provides headspace, while the workflow is optimized around approximately 200 µL of eluate. Nominal capacity and recommended process volume are not the same.

Is it compatible with my magnetic-particle processor?

Compatibility depends on the carrier, tip comb, magnet position and program. Request a drawing and sample, then validate the exact instrument configuration.

Can it be used for PCR thermal cycling?

It is designed as an elution and sample-processing plate. Do not treat it as a PCR plate unless the thermal, optical and sealing requirements have been separately validated.

How can residual volume be minimized?

Use the correct aspirate height, compatible tips, controlled speed and alignment above the V-bottom apex. Optimize these settings using a physical sample.

Are sterile plates available?

Sterile and non-sterile configurations can be discussed. Confirm the required cleanliness documents and packaging before ordering.

Request a Sample, Technical Drawing or Quotation

Tell us the 200 µL elution configuration, intended instrument, working volume, sealing method, required cleanliness status, annual quantity and destination country. Contact Puretest for a sample, current technical drawing, quality-document confirmation or commercial quotation.

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