The Puretest 4.6 mL 48-well square U-bottom deep well plate provides substantially greater volume per position than 96-well formats while retaining a familiar microplate footprint. Its 6 × 8 array is suited to larger-volume sample preparation, mixing, suspension workflows and storage where 48 parallel positions provide the right balance of capacity and throughput.
The Puretest 4.6 mL 48-well square U-bottom deep well plate provides substantially greater volume per position than 96-well formats while retaining a familiar microplate footprint. Its 6 × 8 array is suited to larger-volume sample preparation, mixing, suspension workflows and storage where 48 parallel positions provide the right balance of capacity and throughput.
The plate divides the standard rectangular footprint into 48 square wells arranged in six rows by eight columns. Fewer wells allow substantially more volume at each position, while the U-shaped bases promote fluid circulation and resuspension. This format is useful when a 96-well plate would require splitting one sample across multiple positions.
The plate divides the standard rectangular footprint into 48 square wells arranged in six rows by eight columns. Fewer wells allow substantially more volume at each position, while the U-shaped bases promote fluid circulation and resuspension. This format is useful when a 96-well plate would require splitting one sample across multiple positions.
Because the liquid mass is higher than in 96-well plates, confirm shaker speed, seal strength, centrifuge support and robotic acceleration using the selected fill volume. Do not use the nominal 4.6 mL capacity as the working volume without preserving adequate headspace.
| Plate format | 48 wells; 6 × 8 array |
|---|---|
| Nominal well capacity | 4.6 mL per well |
| Well geometry | Square well; U bottom |
| Material | Natural high-purity polypropylene (PP) |
| Surface | Non-treated |
| Footprint | ANSI/SLAS-style microplate footprint |
| Working-volume guidance | Below nominal capacity; define by mixing, sealing and transport conditions |
| Sealing | Adhesive film, heat seal or 48-well sealing solution; confirm selection |
| Sterility | Sterile and non-sterile supply options subject to quotation |
| Autoclave guidance | Selected configurations: 121°C for 20 minutes; confirm current SKU |
| Cleanliness | Applicable DNase/RNase and pyrogen status confirmed for the quoted SKU |
| Use | Single use; laboratory research and validated sample-processing workflows |
Values are nominal unless a tolerance is stated in the current Puretest drawing. Confirm dimensions, working-volume limits, packaging and applicable quality documentation before equipment validation or a production order.
| Application | Why This Format Is Suitable |
|---|---|
| Larger-volume sample preparation | Processes samples that exceed typical 96-well capacity without splitting positions. |
| Suspension and culture workflows | Provides headspace and U-bottom mixing behavior for validated protocols. |
| Protein and nucleic-acid processing | Handles larger lysis, wash or recovery volumes. |
| Reagent preparation | Organizes multiple buffers or formulations in a plate-compatible format. |
| Sample storage | Combines high per-well capacity with indexed 48-position organization. |
| Automated liquid handling | Can be integrated after validating the carrier, well pitch, tip depth and plate height. |
The plate is manufactured from natural high-purity polypropylene selected for chemical resistance, low biomolecule binding and reliable molding consistency. Available cleanliness and supply configurations should be defined in the quotation. Puretest can confirm the applicable DNase/RNase, pyrogen, sterility and lot-document status for the selected catalog configuration.
If your workflow requires a Certificate of Analysis, Certificate of Conformance, irradiation record, dimensional report, extractables information or a specific contaminant limit, state the requirement before ordering. This ensures that the quoted SKU and batch documentation match your laboratory or regulated purchasing specification.
The plate footprint is designed around common ANSI/SLAS microplate conventions for use with multichannel pipettes, plate handlers and automated liquid-handling platforms. Compatibility still depends on the instrument carrier, gripper, deck position, plate height, tip penetration and software settings; validate the current drawing and a physical sample before routine automation.
Puretest supports samples, technical drawings, sterile and non-sterile supply options, private labels, customer catalog numbers, barcodes, customized pouch and carton artwork, and distributor packaging programs. MOQ, case quantity, carton dimensions, lead time and international shipping method are confirmed with the selected configuration.
| Nominal Format | Well Geometry | Best-Fit Workflow |
|---|---|---|
| 200 µL elution | 96-well square V-bottom | Low-volume nucleic-acid elution and magnetic-bead workflows |
| 1.2 mL | 96-well round U-bottom | Resuspension, sample processing and compact storage |
| 1.6 mL | 96-well square U-bottom | Higher capacity in an automation-friendly footprint |
| 2.0 mL | 96-well round U-bottom | Mixing, recovery and general sample processing |
| 2.2 mL | 96-well U- and V-bottom options | Magnetic-bead processing, high-volume storage and extraction |
| 2.3 mL | 96-well square diamond bottom | Low-dead-volume mixing, automated extraction and high-volume sample processing |
| 4.6 mL | 48-well square U-bottom | Larger-volume culture, mixing and sample preparation |
Choose by required working volume, residual-volume target, mixing method, sealing method and instrument carrier. Request the current drawing before validating an automated protocol.
The 48-well format provides substantially more volume per sample while retaining a plate-based workflow. It is useful when capacity matters more than the maximum number of positions.
4.6 mL is the nominal capacity. Use a lower working volume to preserve headspace for mixing, sealing, heating and transport.
No assumption should be made. Use a sealing solution designed for the 48-well rim layout and confirm it against the current plate drawing.
The footprint supports automation-oriented handling, but 48-well pitch, plate height, carrier support and tip position must be specifically programmed and validated.
Yes, subject to available configuration. Provide the required sterility and documentation expectations when requesting the sample.
Tell us the 4.6 mL configuration, intended instrument, working volume, sealing method, required cleanliness status, annual quantity and destination country. Contact Puretest for a sample, current technical drawing, quality-document confirmation or commercial quotation.
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