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T175 TC-Treated Cell Culture Flask, 0.2 Micron Vent Cap, Sterile

Sterile T175 cell culture flask with a 175 cm2 TC-treated surface, canted neck and orange 0.2 µm vent cap. Recommended medium volume: 40-55 mL.

  • Clear polystyrene
  • Gamma sterilized
  • 5 per bag / 40 per case

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PRODUCT DETAILS

T175 TC-Treated Cell Culture Flask for Higher-Yield Cell Expansion

The Puretest T175 cell culture flask provides a large 175 cm2 TC-treated growth surface for scale-up, higher-yield adherent cell expansion and production-oriented research workflows. Its wide, optically clear polystyrene body supports routine culture observation, while the canted neck improves access for media exchange and cell harvesting. The orange 0.2 µm hydrophobic vent cap provides passive gas exchange during closed-cap incubation.

Product Highlights

  • 175 cm2 TC-treated surface: Offers substantially more culture area for adherent-cell scale-up.
  • 0.2 µm vent cap: Hydrophobic membrane supports continuous gas exchange with the cap closed.
  • Canted neck and broad body: Improve access for serological pipettes, aspiration and cell scrapers.
  • High-clarity polystyrene: Makes media condition and monolayer coverage easier to inspect.
  • Engraved graduations: Provide a convenient visual guide for approximate fill volume.
  • Frosted writing area: Supports clear identification, passage tracking and batch organization.
  • Stackable design: Helps laboratories use incubator and storage space efficiently.
  • Ready for aseptic workflows: Gamma sterilized, non-pyrogenic and DNase/RNase-free as indicated on the product packaging.

Flask Structure and Functional Design

The T175 format combines a broad 175 cm2 TC-treated growth surface with a rigid rectangular body, a canted neck and a 0.2 µm hydrophobic vent-cap membrane. The neck supports access with serological pipettes and cell scrapers, while molded graduations and a frosted writing area aid handling and traceability. Its stackable footprint supports higher-throughput incubator use without changing the familiar flask workflow.

Technical Specifications

Flask format T175
Growth area 175 cm2
Recommended medium volume 40-55 mL
Approx. nominal flask capacity 550 mL
Material Clear polystyrene (PS)
Surface Tissue-culture treated (TC-treated)
Neck Canted neck
Closure Orange 0.2 µm hydrophobic vent cap
Sterility Gamma-irradiation sterilized
Purity Non-pyrogenic; DNase/RNase-free
Pack / case 5 flasks per bag; 40 flasks per case
Use Single use; for laboratory research use only

The 550 mL value is the approximate nominal vessel capacity, not the recommended culture-medium volume. Select the final medium volume for your cell line, seeding density and incubation protocol. Confirm current packing, tolerances and technical drawings with Puretest before ordering.

Application Guide

  • Higher-yield adherent mammalian cell expansion
  • Seed-train and process-development studies
  • Vaccine, virology and recombinant-protein research
  • Cell and gene therapy research workflows
  • Large-batch cell harvesting for downstream assays
  • Scale-up from T25 and T75 culture formats

Why Choose a T175 Flask?

T175 provides a large continuous growth area without moving to multilayer vessels. It is well suited to laboratories that need more cells per vessel, fewer handling steps and an efficient path from routine expansion to higher-yield harvests. For earlier stages of a seed train, see the T25 and T75 formats.

Ordering Information, Packaging and OEM Supply

The T175 flask is supplied sterile in bags of 5, with 40 flasks per case. Current MOQ, lead time and shipping configuration are confirmed in the quotation. Samples may be requested for fit, handling and culture-workflow evaluation. For distribution or high-volume purchasing, ask about private-label, OEM/ODM and custom packaging options.

Request a Sample, Technical Specification or Quotation

Send your required quantity, destination country and intended application through the Puretest contact page.

Frequently Asked Questions

What is the recommended medium volume for a T175 flask?

A typical range is 40-55 mL. The final volume should follow the cell line, seeding density, incubation time and gas-exchange requirements of your protocol.

Is 550 mL the working volume?

No. Approximately 550 mL is the nominal vessel capacity. Routine cell culture uses a lower medium volume spread across the 175 cm2 growth surface.

Is T175 suitable for scale-up?

Yes. T175 is commonly selected after T25 or T75 expansion when more cells are needed per vessel for harvesting or downstream processing.

Can I request samples or private-label packaging?

Yes. Contact Puretest with your estimated annual volume and target market so the team can confirm sample availability, MOQ and suitable OEM/ODM options.

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