T25 TC-Treated Cell Culture Flask, 0.2 Micron Vent Cap, Sterile

T25 TC-Treated Cell Culture Flask for Small-Scale Adherent Cell Culture

The Puretest T25 cell culture flask provides a 25 cm² TC-treated growth surface in a compact, optically clear polystyrene vessel. It is designed for small-volume cell seeding, routine maintenance, clonal expansion and early-stage process development. The canted neck gives convenient pipette and cell-scraper access, while the orange 0.2 µm hydrophobic vent cap supports passive gas exchange during incubation.

Product Highlights

• 25 cm² TC-treated surface: Promotes attachment and spreading of many adherent mammalian cell types.
• 0.2 µm vent cap: Hydrophobic membrane permits continuous gas exchange while helping reduce contamination risk.
• Canted neck: Improves access for pipettes, serological pipettes and cell scrapers.
• High-clarity polystyrene: Supports routine visual inspection and microscope observation.
• Engraved graduations: Make approximate volume checks easier during media addition and harvesting.
• Frosted writing area: Provides space for sample ID, date and passage information.
• Stackable profile: Saves incubator and storage space.
• Ready for aseptic workflows: Gamma sterilized, non-pyrogenic and DNase/RNase-free as indicated on the product packaging.

Flask Structure and Functional Design

The T25 format combines a flat 25 cm² TC-treated growth surface with a compact rectangular body, a canted neck and a 0.2 µm hydrophobic vent-cap membrane. The neck geometry supports controlled access with serological pipettes and cell scrapers, while engraved graduations and a frosted writing area assist routine handling and traceability. Its stackable profile is designed for efficient incubator use.

Technical Specifications

The 50 mL value is the approximate nominal vessel capacity, not the recommended culture-medium volume. Select the final medium volume for your cell line, seeding density and incubation protocol. Confirm current packing, tolerances and technical drawings with Puretest before ordering.

Application Guide

• Small-scale adherent cell maintenance and expansion
• Cell-line screening and method development
• Transfection, infection and expression studies
• Primary cell and stem-cell research
• Preparation of cells for microscopy or downstream assays
• Seed cultures before scale-up to T75 or T175 flasks

Why Choose a T25 Flask?

T25 is a practical starting format when cell numbers are limited, media cost matters or several conditions need to be handled in parallel. Its small footprint is convenient for cell recovery, protocol optimization and maintenance cultures. When more biomass is required, the same workflow can be moved to our T75 or T175 cell culture flasks.

Ordering Information, Packaging and OEM Supply

The T25 flask is supplied sterile in bags of 10, with 200 flasks per case. Current MOQ, lead time and shipping configuration are confirmed in the quotation. Samples may be requested for fit, handling and culture-workflow evaluation. For distribution or high-volume purchasing, ask about private-label, OEM/ODM and custom packaging options.

Request a Sample, Technical Specification or Quotation

Send your required quantity, destination country and intended application through the Puretest contact page.

Frequently Asked Questions

What is the recommended medium volume for a T25 flask?

A typical range is 5-10 mL. The optimum volume depends on the cell type, gas-exchange requirement, incubation time and protocol.

What does TC-treated mean?

TC treatment modifies the polystyrene growth surface to increase hydrophilicity, helping many anchorage-dependent cells attach and spread more consistently than on untreated polystyrene.

Does the vent cap need to be loosened in the incubator?

No. The 0.2 µm hydrophobic membrane is designed to permit passive gas exchange with the cap closed. Follow your laboratory SOP for incubator use and aseptic handling.

Can I order samples or private-label packaging?

Yes. Contact Puretest with your estimated annual volume and target market so the team can confirm sample availability, MOQ and suitable OEM/ODM options.