Sterile 48-well TC-treated polystyrene plate for efficient screening, optimization and replicate-heavy cell studies. Lower working volume reduces media and compound use per condition.
The Puretest 48-well TC-treated cell culture plate increases experimental capacity while using less medium and reagent per condition than 6-, 12- or 24-well formats. Each well provides approximately 0.95 cm2 of growth area, making the plate a practical choice for screening, optimization and replicate-heavy studies that still require convenient manual access and useful cell yield.
The 6 x 8 plate combines a uniform tissue-culture-treated surface, clear flat well bottoms and a matching lid. It is suited to dose-response testing, media and coating optimization, cell viability studies, cytotoxicity assays, microscopy and image-based analysis. Each plate is gamma-irradiation sterilized and individually sealed for aseptic preparation.
The plate uses 48 individually separated wells arranged in a 6 x 8 layout. Each well has an approximate diameter of 11.0 mm, an approximate growth area of 0.95 cm2 and an approximate total volume of 1.6 mL. A typical culture-medium range is approximately 0.19-0.285 mL per well, supporting efficient surface coverage while conserving media and test compounds.
Compared with a 96-well plate, the 48-well format gives operators more room for manual pipetting, washing and imaging. It is often selected during assay development before a workflow is transferred to a higher-throughput 96-well format.
| Plate format | 48 wells |
|---|---|
| Well layout | 6 x 8 |
| Material | High-clarity polystyrene (PS) |
| Surface treatment | Tissue-culture treated (TC-treated) |
| Bottom | Clear, flat bottom |
| Approx. growth area | 0.95 cm2 per well |
| Typical working volume | 0.19-0.285 mL per well |
| Approx. total well volume | 1.6 mL per well |
| Approx. well diameter | 11.0 mm |
| Approx. plate footprint | 127.8 x 85.5 mm |
| Lid | Included; designed for covered incubation and handling |
| Sterilization | Gamma irradiation |
| Cleanliness profile | Non-pyrogenic; DNase/RNase-free |
| Primary packaging | 1 plate per individual sealed pouch |
| Use | Single use; for laboratory research use only |
| Recommended storage | 5-30°C; keep dry and away from direct sunlight |
| Shelf life | 3 years from date of manufacture when stored as directed |
Dimensions and volumes are nominal. Confirm the current technical drawing, tolerances, packaging quantity and applicable quality documentation with Puretest before placing a production order or validating automated equipment.
Untreated polystyrene is naturally hydrophobic and may not provide reliable attachment for many anchorage-dependent cells. Tissue-culture treatment improves surface hydrophilicity, helping many adherent cells attach, spread and form more consistent monolayers.
This plate is intended primarily for adherent-cell workflows. Suspension cells, low-attachment models and specialized assays may require a non-treated or application-specific surface.
| Application | Why the 48-Well Format Is Suitable |
|---|---|
| Dose-response screening | Supports more concentration points and replicates while reducing compound and medium use. |
| Cell viability or cytotoxicity assays | Balances throughput with enough well area for routine observation and endpoint analysis. |
| Media and coating optimization | Enables efficient comparison of multiple formulations and surface conditions. |
| Transfection optimization | Allows reagent-ratio and dose matrices with lower material consumption. |
| Microscopy and image analysis | Clear flat bottoms support routine imaging with compatible instruments. |
| Replicate-heavy study design | Provides enough wells for controls, technical replicates and several treatment groups. |
| Assay transfer and development | Offers an intermediate step between manually accessible 24-well and higher-throughput 96-well formats. |
The approximately standard microplate footprint is suitable for many workflows, including standard CO2 incubators, compatible inverted microscopes and imaging systems, manual and multichannel pipetting, plate shakers that accept the plate dimensions and selected automated liquid-handling platforms.
For optical or automated use, confirm plate-carrier fit, focal requirements, deck compatibility and handling parameters using the current product drawing.
These recommendations are general guidance. Users should follow their validated cell-culture protocol and institutional biosafety procedures.
The plate is sterilized by gamma irradiation and individually sealed. Depending on the specification and order requirements, quality-control activities may include raw-material and appearance inspection, dimensional checks, surface-treatment consistency, packaging-integrity inspection, sterility validation, DNase/RNase testing, pyrogen or endotoxin evaluation and cell-attachment performance assessment.
If your purchasing specification requires a particular Sterility Assurance Level, endotoxin limit, Certificate of Analysis, Certificate of Conformance or other batch document, state the requirement before ordering. Puretest will confirm which limits and documents apply to the quoted product and production lot.
Each plate is individually sealed for convenient single-plate access. Inner-pack quantity, case quantity, carton dimensions, gross weight, MOQ and lead time are confirmed in the quotation. Export cartons are prepared to help protect products from compression and packaging damage during transportation.
Orders can be arranged by courier, air or sea according to delivery urgency, destination and volume. Samples can be requested for handling, cell-attachment and assay evaluation.
Subject to order quantity and technical review, available services may include private-label sterile pouches and cartons, customized labels and barcodes, customer catalog numbers, tailored pack quantities, branded instruction sheets, multilingual packaging, batch documentation and long-term distributor supply planning.
For an OEM proposal, provide the target market, estimated annual volume, packaging language, delivery schedule and documentation requirements.
| Format | Relative Culture Area | Reagent Use | Typical Use |
|---|---|---|---|
| 6-well | Largest | Highest | Expansion, coverslips, scraping and higher-yield collection |
| 12-well | Medium-large | Medium | Transfection, staining, optimization and sample preparation |
| 24-well | Medium | Medium-low | Multi-condition assays, microscopy and dose-response studies |
| 48-well | Small | Low | Screening, optimization and replicate-heavy studies |
| 96-well | Smallest | Lowest | Higher-throughput cell assays and parallel screening |
It doubles the condition capacity of a 24-well plate while retaining more manual access and well area than a 96-well plate.
Approximately 0.19-0.285 mL per well is a typical starting range. Optimize volume for the cell type, incubation time and evaporation conditions.
The 6 x 8 layout can support compatible multichannel workflows. Confirm channel spacing, tip position and protocol parameters before routine use.
The TC-treated surface is primarily designed for adherent cells. Ask Puretest whether a non-treated or specialized surface is more appropriate.
The clear flat bottoms support routine microscopy and imaging. Confirm instrument optics and focal requirements before ordering.
Yes. Include the cell type, assay, quantity and destination country with the sample request.
Applicable batch and quality documents can be provided according to the quoted product and order requirements. Confirm the required list before ordering.
Private-label packaging, labels, barcodes, instruction sheets and carton markings can be discussed for eligible volume orders.
Tell us your cell type, intended workflow, annual quantity, destination country and packaging requirements. Contact Puretest for a sample, current technical drawing, applicable quality documentation or commercial quotation.
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