Sterile 12-well TC-treated polystyrene plate for adherent-cell culture, transfection, staining and microscopy. The 3 x 4 format combines approximately 3.8 cm2 growth area per well with efficient parallel testing.
The Puretest 12-well TC-treated cell culture plate is designed for parallel experiments that need more culture area and sample volume than 24- or 48-well formats. Each well provides approximately 3.8 cm2 of growth area, giving researchers enough space for cell recovery, imaging and downstream sample preparation while still allowing 12 conditions to be processed on one plate.
The clear polystyrene plate combines a tissue-culture-treated growth surface, flat transparent well bottoms and a matching lid. It is suited to routine adherent-cell culture, transfection optimization, immunocytochemistry, dose-response studies and coverslip-based workflows. Each plate is gamma-irradiation sterilized and supplied in an individual sealed pouch for convenient aseptic access.
The plate uses a standard rectangular microplate footprint with 12 individually separated wells in a 3 x 4 arrangement. Each well has an approximate diameter of 22.1 mm and an approximate total volume of 6.9 mL. For routine cell culture, a working range of approximately 0.76-1.14 mL per well provides surface coverage while helping control reagent consumption. Final volume should always be optimized for the selected cell line, incubation period and evaporation conditions.
The transparent flat bottom supports direct observation with compatible inverted microscopes. The wider wells can also accommodate selected round coverslips for staining and microscopy; buyers should confirm the coverslip diameter against the technical drawing before use.
| Plate format | 12 wells |
|---|---|
| Well layout | 3 x 4 |
| Material | High-clarity polystyrene (PS) |
| Surface treatment | Tissue-culture treated (TC-treated) |
| Bottom | Clear, flat bottom |
| Approx. growth area | 3.8 cm2 per well |
| Typical working volume | 0.76-1.14 mL per well |
| Approx. total well volume | 6.9 mL per well |
| Approx. well diameter | 22.1 mm |
| Approx. plate footprint | 127.8 x 85.5 mm |
| Lid | Included; designed for covered incubation and handling |
| Sterilization | Gamma irradiation |
| Cleanliness profile | Non-pyrogenic; DNase/RNase-free |
| Primary packaging | 1 plate per individual sealed pouch |
| Use | Single use; for laboratory research use only |
| Recommended storage | 5-30°C; keep dry and away from direct sunlight |
| Shelf life | 3 years from date of manufacture when stored as directed |
Dimensions and volumes are nominal. Confirm the current technical drawing, tolerances, packaging quantity and applicable quality documentation with Puretest before placing a production order or validating automated equipment.
Untreated polystyrene is naturally hydrophobic and may not provide suitable attachment for many anchorage-dependent cells. Tissue-culture treatment modifies the polystyrene surface to improve hydrophilicity, helping many adherent cells attach, spread and form more consistent monolayers.
This plate is primarily intended for adherent-cell workflows. Suspension cells, low-attachment models and specialized assays may require a non-treated or application-specific surface. Contact Puretest if you need help choosing the appropriate surface.
| Application | Why the 12-Well Format Is Suitable |
|---|---|
| Adherent cell culture | Provides useful growth area for routine maintenance, expansion and cell recovery. |
| Transfection optimization | Allows multiple reagent ratios, nucleic-acid doses or treatment conditions on one plate. |
| Immunocytochemistry | Supports cell staining and observation directly in the wells or on compatible coverslips. |
| Dose-response studies | Enables parallel comparison of multiple treatment concentrations with practical sample volume. |
| Time-course experiments | Provides enough wells to allocate several collection points and controls. |
| Coverslip culture | The well diameter may accommodate compatible round coverslips for imaging workflows. |
| Downstream sample preparation | Provides practical cell yield for protein, RNA, DNA or other validated analyses. |
The approximately standard microplate footprint is suitable for many common laboratory workflows, including:
Instrument compatibility should be confirmed using the current product drawing and the instrument manufacturer’s dimensional requirements before automated use.
These recommendations are general guidance. Users should follow their validated cell-culture protocol and institutional biosafety procedures.
The plates are sterilized by gamma irradiation and individually sealed to support aseptic handling. Depending on the product specification and order requirements, quality-control activities may include raw-material and appearance inspection, dimensional checks, surface-treatment consistency, packaging-integrity inspection, sterility validation, DNase/RNase testing, pyrogen or endotoxin evaluation and cell-attachment performance assessment.
If your purchasing specification requires a particular Sterility Assurance Level, endotoxin limit, Certificate of Analysis, Certificate of Conformance or other batch document, state that requirement before ordering. Puretest will confirm which limits and documents apply to the quoted product and production lot. This avoids relying on generic claims that may not match your regulated or validated workflow.
Each plate is individually sealed for convenient single-plate access. Inner-pack quantity, case quantity, carton dimensions, gross weight, MOQ and lead time are confirmed in the commercial quotation so the shipment matches the selected packaging configuration. Export cartons are prepared to help protect products from compression and packaging damage during transit.
Orders can be arranged by courier, air or sea according to delivery urgency, destination and volume. Buyers can request samples before bulk purchasing to evaluate plate handling, cell attachment and compatibility with their own protocols.
Puretest supports laboratory distributors, consumables brands and volume buyers. Subject to order quantity and technical review, available services may include:
For an OEM proposal, provide the target market, estimated annual volume, required packaging language, desired delivery schedule and applicable documentation requirements.
| Format | Relative Culture Area | Reagent Use | Typical Use |
|---|---|---|---|
| 6-well | Larger | Higher | Cell expansion, coverslip work and higher-yield collection |
| 12-well | Medium-large | Medium | Transfection, staining, optimization and sample preparation |
| 24-well | Medium | Medium-low | Multi-condition cell assays and microscopy |
| 48-well | Small | Low | Screening and assay development |
| 96-well | Smaller | Lower | Higher-throughput cell assays and parallel screening |
The TC-treated surface is primarily designed for adherent-cell culture. For suspension-cell, low-attachment or specialized assays, contact Puretest to confirm whether a non-treated or application-specific plate is more appropriate.
Approximately 0.76-1.14 mL per well is a typical starting range. Optimize the final volume for the cell type, incubation duration, gas exchange and evaporation control required by your protocol.
The approximate 22.1 mm well diameter may accommodate selected round coverslips. Confirm the coverslip diameter, plate drawing and handling method before use.
Yes. Samples can be requested for evaluation. Include your cell type, intended application, quantity and destination country so the team can recommend a suitable sample arrangement.
Applicable quality and batch documents can be provided according to the quoted product and order requirements. Confirm the required document list, test limits and regulatory expectations before ordering.
Private-label pouches, labels, barcodes, instruction sheets and carton markings can be discussed for eligible volume orders.
The plate uses an approximately standard microplate footprint, but compatibility depends on the instrument, carrier, gripper, deck layout and software settings. Validate compatibility using the current technical drawing before routine automated use.
Please provide the required quantity, destination country, intended application, preferred packaging, expected delivery time and any quality-document or OEM requirements.
Tell us your cell type, intended workflow, annual quantity, destination country and packaging requirements. Contact Puretest to request a sample, current technical drawing, applicable quality documentation or commercial quotation.
General Inquiries
Enquiry for: 12-Well TC-Treated Cell Culture Plate, Flat Bottom, Sterile
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