Sterile 24-well TC-treated polystyrene plate for dose-response studies, transfection, staining and microscopy. The format balances useful growth area with efficient parallel testing.
The Puretest 24-well TC-treated cell culture plate is designed for researchers who need more experimental conditions than 6- or 12-well formats without moving to very small assay volumes. Each well provides approximately 1.9 cm2 of growth area, offering a practical balance between throughput, microscopy access, cell recovery and reagent consumption.
The 4 x 6 plate combines a uniform tissue-culture-treated surface, clear flat well bottoms and a matching lid. It is well suited to dose-response studies, transfection optimization, immunocytochemistry, fluorescence imaging, coverslip culture and multi-condition cell assays. Each plate is gamma-irradiation sterilized and individually sealed for convenient aseptic preparation.
The plate uses 24 individually separated wells arranged in a 4 x 6 layout. Each well has an approximate diameter of 15.6 mm, an approximate growth area of 1.9 cm2 and an approximate total volume of 3.4 mL. A typical culture-medium range is approximately 0.38-0.57 mL per well, allowing efficient surface coverage while reducing reagent use compared with lower-density formats.
The format provides enough well area for cell observation, washing, staining and selected sample-recovery procedures. It is often chosen for experiments that require concentration series, controls and replicates on the same plate without the smaller working volumes of 48- or 96-well formats.
| Plate format | 24 wells |
|---|---|
| Well layout | 4 x 6 |
| Material | High-clarity polystyrene (PS) |
| Surface treatment | Tissue-culture treated (TC-treated) |
| Bottom | Clear, flat bottom |
| Approx. growth area | 1.9 cm2 per well |
| Typical working volume | 0.38-0.57 mL per well |
| Approx. total well volume | 3.4 mL per well |
| Approx. well diameter | 15.6 mm |
| Approx. plate footprint | 127.8 x 85.5 mm |
| Lid | Included; designed for covered incubation and handling |
| Sterilization | Gamma irradiation |
| Cleanliness profile | Non-pyrogenic; DNase/RNase-free |
| Primary packaging | 1 plate per individual sealed pouch |
| Use | Single use; for laboratory research use only |
| Recommended storage | 5-30°C; keep dry and away from direct sunlight |
| Shelf life | 3 years from date of manufacture when stored as directed |
Dimensions and volumes are nominal. Confirm the current technical drawing, tolerances, packaging quantity and applicable quality documentation with Puretest before placing a production order or validating automated equipment.
Untreated polystyrene is naturally hydrophobic and may not support reliable attachment of many anchorage-dependent cells. Tissue-culture treatment improves surface hydrophilicity, helping many adherent cells attach, spread and form more consistent monolayers.
The plate is intended primarily for adherent-cell workflows. Suspension cells, low-attachment models and specialized assays may require a non-treated or application-specific surface.
| Application | Why the 24-Well Format Is Suitable |
|---|---|
| Dose-response studies | Supports concentration series, controls and replicates while keeping medium use manageable. |
| Transfection optimization | Allows multiple reagent ratios or nucleic-acid doses with enough cells for imaging and analysis. |
| Immunocytochemistry | Clear flat bottoms support staining and observation directly in the wells. |
| Fluorescence microscopy | Provides a practical imaging area and may accommodate compatible coverslips. |
| Cell viability or cytotoxicity studies | Balances replicate capacity with useful culture area and sample volume. |
| Tissue-explant or primary-cell culture | Offers more conditions than larger-well plates while retaining manual handling access. |
| Media or coating optimization | Enables side-by-side comparison of formulations, coatings and incubation conditions. |
The approximately standard microplate footprint is suitable for many common workflows, including standard CO2 incubators, compatible inverted microscopes, manual or multichannel pipetting, plate shakers that accept the plate dimensions, staining and imaging procedures and selected automated liquid-handling platforms.
For instrument-based use, confirm carrier fit, optical requirements and automation compatibility using the current product drawing.
These recommendations are general guidance. Users should follow their validated cell-culture protocol and institutional biosafety procedures.
The plate is sterilized by gamma irradiation and individually sealed. Depending on the specification and order requirements, quality-control activities may include raw-material and appearance inspection, dimensional checks, surface-treatment consistency, packaging-integrity inspection, sterility validation, DNase/RNase testing, pyrogen or endotoxin evaluation and cell-attachment performance assessment.
If your specification requires a particular Sterility Assurance Level, endotoxin limit, Certificate of Analysis, Certificate of Conformance or other batch document, state the requirement before ordering. Puretest will confirm which limits and documents apply to the quoted product and lot.
Each plate is individually sealed for convenient single-plate access. Inner-pack quantity, case quantity, carton dimensions, gross weight, MOQ and lead time are confirmed in the quotation. Export cartons are prepared to help protect products from compression and packaging damage during transportation.
Orders can be arranged by courier, air or sea according to delivery urgency, destination and volume. Samples can be requested for handling, cell-attachment and workflow evaluation.
Subject to order quantity and technical review, available services may include private-label sterile pouches and cartons, customized labels and barcodes, customer catalog numbers, tailored pack quantities, branded instruction sheets, multilingual packaging, batch documentation and long-term distributor supply planning.
For an OEM proposal, provide the target market, estimated annual volume, packaging language, delivery schedule and documentation requirements.
| Format | Relative Culture Area | Reagent Use | Typical Use |
|---|---|---|---|
| 6-well | Largest | Highest | Expansion, coverslips, scraping and higher-yield collection |
| 12-well | Medium-large | Medium | Transfection, staining, optimization and sample preparation |
| 24-well | Medium | Medium-low | Multi-condition assays, microscopy and dose-response studies |
| 48-well | Small | Low | Screening and assay development |
| 96-well | Smallest | Lowest | Higher-throughput cell assays and parallel screening |
It provides more conditions and replicates than 6- or 12-well plates while retaining enough growth area for microscopy, coverslips and selected cell-recovery workflows.
Approximately 0.38-0.57 mL per well is a typical starting range. Optimize volume for the cell type, incubation time and evaporation control required by your protocol.
The approximate 15.6 mm well diameter may accommodate selected round coverslips. Confirm diameter and handling method before use.
The TC-treated surface is primarily designed for adherent cells. Ask Puretest whether a non-treated or specialized surface is more suitable for suspension workflows.
The clear flat-bottom wells support routine microscopy and imaging. Confirm the optical requirements of your instrument and assay before ordering.
Yes. Include the cell type, intended application, quantity and destination country with your sample request.
Applicable batch and quality documents can be provided according to the quoted product and order requirements. Confirm the required list before ordering.
Private-label packaging, labels, barcodes, instruction sheets and carton markings can be discussed for eligible volume orders.
Tell us your cell type, intended workflow, annual quantity, destination country and packaging requirements. Contact Puretest for a sample, current technical drawing, applicable quality documentation or commercial quotation.
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Enquiry for: 24-Well TC-Treated Cell Culture Plate, Flat Bottom, Sterile
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