20 µL filtered OT-2-compatible pipette tips for low-volume, contamination-conscious automated liquid handling.
Puretest 20 µL filtered pipette tips are designed as an independent compatible consumable for low-volume liquid handling on Opentrons OT-2 robots equipped with current P20 GEN2 single-channel or 8-channel pipettes. The clear polypropylene tip and integrated aerosol barrier support routine molecular-biology and assay-setup workflows where both low-volume control and contamination protection matter.
The tip geometry, upper cone, distal orifice and 96-position rack work as one mechanical system. Consistent molding is therefore important not only for liquid transfer, but also for reliable multichannel pickup, repeatable nozzle sealing, deck clearance and clean tip ejection.
Important: These are independent compatible tips, not original Opentrons consumables. Instrument, rack and protocol performance must be qualified on the exact OT-2 configuration before routine or regulated use.
At low microliter volumes, small variations in tip-cone seal, internal taper or orifice geometry can create proportionally large transfer errors. The rigid polypropylene body is designed to maintain a stable seal while the narrow distal section supports controlled aspiration and dispense. The filter is positioned above the normal working liquid column and should not be intentionally wetted.
Because the OT-2 8-channel pipette picks up an entire row simultaneously, rack flatness and tip-height consistency are especially important. Qualification should include all eight channels, partial and full rack conditions, representative source/destination labware and the liquid classes used in the final protocol.
| Parameter | Specification | Selection note |
|---|---|---|
| Nominal tip capacity | 20 µL | Use the smallest tip that covers the required transfer volume. |
| Recommended working range | Approximately 1–20 µL | Final limits depend on the exact pipette, liquid class and method validation. |
| Tip material | Clear, non-conductive polypropylene | Not intended for capacitive liquid-level sensing. |
| Barrier | Hydrophobic aerosol filter | Do not over-aspirate or wet the filter. |
| Rack format | 96 positions, ANSI/SLAS footprint | Confirm rack definition, deck slot and pickup height. |
| Primary pipettes | OT-2 P20 GEN2, 1-channel and 8-channel | GEN1 or legacy use requires separate physical and method qualification. |
| Autoclaving | Not recommended | Use the supplied condition and documented storage instructions. |
| Item | Compatibility guidance | What to verify |
|---|---|---|
| Robot | Opentrons OT-2 | Robot generation, deck configuration and current software method. |
| Pipettes | P20 GEN2 single-channel and 8-channel | Nozzle fit, full-row pickup, seal and ejection. |
| Tip definition | 20 µL filtered OT-2-compatible rack definition | Labware name, dimensions, tip length and Z offsets. |
| Cross-platform use | Not interchangeable with Opentrons Flex tips | Keep OT-2 and Flex inventory clearly segregated. |
This page covers the 20 µL filtered configuration. For protocols that do not require an aerosol barrier, use the separate 20 µL non-filtered rack/refill page. Filtered tips are normally supplied as complete racks; confirm the exact packaging and sterility status on the quotation.
| Configuration | Best suited to | Supply / qualification note |
|---|---|---|
| 20 µL filtered rack | PCR/qPCR setup, NGS, diagnostic sample preparation and contamination-sensitive transfers | 96 tips per rack; confirm sterile/purity documentation. |
| 20 µL non-filtered rack/refill | Routine reagent transfer where aerosol protection is not required | Available on the separate non-filtered product page. |
To receive the correct quotation, specify: OT-2 robot generation, P20 pipette model and channel count, 20 µL filtered configuration, rack quantity, sterility/purity requirements, required documents and destination market. Puretest supports evaluation samples, distributor supply, recurring bulk orders and OEM/private-label programs for confirmed configurations.
No. They are independent compatible consumables and must be qualified on the exact OT-2 system and protocol.
Use 1–20 µL as the initial selection range, then verify accuracy and precision at the minimum, typical and maximum volumes used in your method.
No. Over-aspiration into the filter can reduce recovery and may invalidate the transfer.
No. The two platforms use different tip and rack interfaces and should be treated as separate consumable families.
Yes. Provide the instrument, pipette and protocol details so the correct configuration and available documents can be matched.
Send your robot model, pipette model, channel count, target volume, filtered/non-filtered preference, rack/refill preference, sterility requirement and estimated annual demand. We will match the most appropriate configuration and provide available qualification documents.
Contact Puretest for an evaluation sample or quotation
Opentrons and the related product names are trademarks of their respective owner. Puretest is an independent compatible-consumables supplier and is not affiliated with or endorsed by Opentrons Labworks, Inc.
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