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Optical qPCR Sealing Film

Choose an optical plate seal around your qPCR instrument, reaction volume and plate surface. This family brings pressure-sensitive optical films together in one place, with initial-tack, low-tack and no-initial-tack options for different handling preferences.

  • Optical assay selection: Intended for workflows that read fluorescence through the seal; assess the exact film with your detection channels.
  • Pressure-sensitive construction: Controlled pressure helps the sealing layer contact the rim around each reaction well.
  • Handling choices: Compare initial adhesion for easy placement versus repositioning before final pressing.

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PRODUCT DETAILS

Choose an optical plate seal around your qPCR instrument, reaction volume and plate surface. This family brings pressure-sensitive optical films together in one place, with initial-tack, low-tack and no-initial-tack options for different handling preferences.

Product Highlights

  • Optical assay selection. Intended for workflows that read fluorescence through the seal; assess the exact film with your detection channels.
  • Pressure-sensitive construction. Controlled pressure helps the sealing layer contact the rim around each reaction well.
  • Handling choices. Compare initial adhesion for easy placement versus repositioning before final pressing.
  • Individual sheets. Liner-backed sheets and handling tabs support routine manual plate preparation.

Structure and Functional Design

Component Function
Clear continuous film Covers the well array without intentionally open pores or pre-cut access slits.
Sealing layer Needs even contact with every well rim; pressing only the outer perimeter is not sufficient.
Liner and handling tabs Allow positioning while keeping fingers away from the active sealing surface.

Technical Specifications and Selection Guide

Use the configurations below to specify your inquiry. Dimensions and thicknesses are reference configurations, not a guarantee of stock or universal compatibility. The selected grade, usable sealing area, packaging and operating limits will be confirmed in the quotation and product specification.

Option Nominal thickness Sheet size (mm) Selection note
Initial-tack polyolefin 100 ± 10 µm 130 × 80; 137 × 80; 140 × 80 Initial adhesion helps retain placement before final pressing.
No-initial-tack polyolefin 100 ± 10 µm 140 × 80 Position first, then apply consistent sealing pressure.
Low-tack polyolefin 110 ± 10 µm 141 × 80 Lower initial tack aids alignment; confirm final adhesion on your plate.
Item Selection information
Format Individual adhesive/pressure-sensitive optical sheets
Reference pack 100 sheets/box, subject to selected grade
Plate fit Confirm outer dimensions, usable sealing area and tab clearance
Qualification Instrument optics, plate resin, cycling program and reaction volume

Applications

For real-time PCR method development and routine fluorescence-based amplification after plate-and-instrument qualification. Compare seals using your actual assay rather than relying on visual clarity alone.

Application and Compatibility Checklist

  1. Start with a clean, dry plate rim and a fully thawed, room-temperature film unless the selected grade instructs otherwise.
  2. Align the clear sealing area over all wells. Keep tabs clear of the heated lid and instrument transport mechanism.
  3. Press evenly across the well array with a suitable applicator, paying particular attention to corners and perimeter wells.
  4. Run an assay comparison with positive and negative controls. Check evaporation, fluorescence background and edge-well behavior before routine use.
Selection note: A clear appearance alone does not establish qPCR compatibility. The existing polyester optical construction and the polyolefin pressure-sensitive options are different grades; do not substitute one for the other without confirmation.

Product Image Notes

Pressure-sensitive optical film shown on a demonstration plate. Circular marks on lifted film are sealing impressions, not perforations. Plate and applicators are not included.

Ordering Information

For a useful quotation, send your plate model or drawing, preferred film construction, sheet size or roll-feed requirements, application conditions, quantity and destination country. For heat-seal products, include the sealer model. If lot-specific quality documents or special packaging are required, list them in the inquiry so they can be checked for the selected grade.

Evaluate a representative sample under your actual workflow before changing a qualified laboratory consumable. A general family description does not replace grade-specific test data.

Frequently Asked Questions

Which tack level should I choose?

Choose around your plate-positioning workflow. Initial tack, low tack and no initial tack describe placement behavior, not a ranking of final assay performance.

Does 140 × 80 mm guarantee 96-well or 384-well compatibility?

No. Overall sheet size may include handling tabs. Confirm the usable sealing area, well-rim design and instrument clearance.

Can you provide optical or nuclease documentation?

State the documents required in your inquiry. Optical measurements, sterility and nuclease declarations must relate to the exact supplied grade and lot.

Compare Related Sealing Options

Find the seal that fits your workflow

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