HPLC or high-performance liquid chromatography is a chromatography technique that utilizes different vials and containers, including:
– Autosampler vials – Small vials made of glass or plastic that hold samples for automatic injection into the HPLC system. They come in various volumes like 1.5 mL, 2 mL, etc.
– Mobile phase reservoirs – Larger containers that hold the aqueous and/or organic mobile phases used to carry the samples through the chromatographic column. Often made of glass or polyethylene.
– Waste vials – Collect the used mobile phase and sample after passing through the column. Typically glass or plastic.
– Fraction collector vials – Collect separated sample components exiting the column over time. Allow capturing pure compounds from a sample mixture. Various sizes from 0.5 mL up to 100 mL.
– Standard vial – Hold reference standards for calibration and quantification. Usually glass with volumes like 2 mL or 10 mL.- Sample filtration vials – Hold samples and allow filtering into autosampler vials. Help remove particulates that could clog the system.
The specific vials used depend on the HPLC system, samples, and application. But these are some of the common vial types employed in HPLC workflows. Proper vial selection helps ensure good chromatographic runs.
