Use Case of Cell Culture Flask

Here are some common use cases and applications of cell culture flasks:

– Routine cell culture – Flasks provide growth surfaces for anchorage-dependent cell lines and the production of biomass. Different sizes are available based on scale needs.

– Cell line maintenance – Flasks allow routine sub-culturing and maintenance of cells to preserve genotype and phenotype.

– Primary cell cultures – Culturing of primary cells obtained from tissues utilizes flasks for expansion and characterization.

– Cell-based assays – Bioassays assessing growth, viability, cytotoxicity, etc. are performed in flasks.

– Virus production – Flasks enable the growth of virus stocks in cell culture for use in research or vaccines.

– Hybridoma generation – Fusing antibody-producing B-cells with myeloma cells to produce hybridomas is done in flasks.

– Culture of stem cells – Flasks provide specialized growth surfaces to expand embryonic or adult stem cells while retaining pluripotency.

– Conditioned media preparation – Collecting cell-secreted factors in conditioned media for further study requires flasks.

– Transfection experiments – Flasks allow transfection and selection of transformed cell lines expressing recombinant proteins.

– Tumor spheroid culture – 3D tumor models are grown in flasks using scaffolds or as suspension spheroids.

– Cell differentiation studies – Inducing cell differentiation along specific lineages is facilitated in flasks.

– Cell-based immunotherapy – Flasks enable the expansion of therapeutic cells like T-cells, dendritic cells, etc.

So in summary, cell culture flasks provide versatile surfaces for attachment and growth of diverse cell types for research and bioproduction applications.

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