T75 TC-Treated Cell Culture Flask for Routine Cell Expansion

The Puretest T75 cell culture flask combines a 75 cm² tissue-culture-treated surface with a clear, space-efficient polystyrene body. It is a versatile format for routine expansion of adherent cells, medium-scale assay preparation and seed-train development. A canted neck improves liquid transfer and scraper access, and the orange 0.2 µm hydrophobic vent cap supports passive gas exchange while the flask remains closed.

Key Features

• 75 cm² TC-treated growth surface: Suitable for dependable attachment and growth of many adherent cell lines.
• 0.2 µm vent cap: Hydrophobic membrane supports continuous gas exchange and closed-cap incubation.
• Canted-neck design: Helps simplify media addition, aspiration and cell harvesting.
• Clear polystyrene construction: Enables convenient visual inspection of media and cell monolayers.
• Dual-side graduations: Support quick approximate volume assessment.
• Frosted writing zone: Makes sample identification and passage tracking easier.
• Stable, stackable shape: Uses incubator, biosafety-cabinet and storage space efficiently.
• Cleanliness profile: Gamma sterilized, non-pyrogenic and DNase/RNase-free as indicated on the product packaging.

Technical Specifications

The 175 mL value is the approximate nominal vessel capacity, not the recommended culture-medium volume. Select the final medium volume for your cell line, seeding density and incubation protocol. Confirm current packing, tolerances and technical drawings with Puretest before ordering.

Typical Applications

• Routine adherent mammalian cell expansion
• Vaccine, virology and recombinant-protein research
• Transfection and gene-expression workflows
• Stem-cell, primary-cell and cancer-cell research
• Seed culture preparation before larger-scale expansion
• Cell harvesting for molecular and biochemical assays

Why Choose a T75 Flask?

T75 offers a useful balance between culture area, medium consumption and incubator footprint. It can provide more cells than a T25 without the handling and media requirements of a T175, making it a common format for routine passage work and pre-assay expansion. For smaller or larger workflows, compare our T25 and T175 flasks.

Packaging, Samples and OEM Supply

The T75 flask is supplied sterile in bags of 5, with 100 flasks per case. Current MOQ, lead time and shipping configuration are confirmed in the quotation. Samples may be requested for fit, handling and culture-workflow evaluation. For distribution or high-volume purchasing, ask about private-label, OEM/ODM and custom packaging options.

Request a sample or quotation: Send your required quantity, destination country and intended application through the Puretest contact page.

Frequently Asked Questions

What is the recommended medium volume for a T75 flask?

A typical range is 15-25 mL. The best volume depends on the cell type, gas-exchange requirement, incubation time and laboratory protocol.

Is 175 mL the working volume?

No. Approximately 175 mL is the nominal vessel capacity. Routine cell culture generally uses a much lower medium volume distributed across the 75 cm² growth surface.

How does the vent cap work?

The 0.2 µm hydrophobic membrane allows passive gas exchange while the cap remains closed. This supports incubator use and helps reduce unnecessary handling.

Can I request samples or private-label packaging?

Yes. Contact Puretest with your estimated annual volume and target market so the team can confirm sample availability, MOQ and suitable OEM/ODM options.